Finished grants:

• Effect of exogenous chemical stress on precesses in yeast mambrane studied by means of fluorescent probes

  Grant agency: GAUK
  Identification number of grant: GAUK 135109
  Principal investigator: RNDr. Andrea Drietomská
  Co-Principal investigators: doc. RNDr. Dana Gášková, CSc.
  
  
  Grant annotation

  The project focuses od studying mechanisms of activity of membrane transporters in intact cells of the yeast Saccharomyces cerevisiae and response of these cells to the chemical stress. Our main concern is the study of a) MDR (Multidrug resistance) pumps, transport proteins located in cytoplasmic membrane, responsible for cells´ resistance against a wide variety of compounds (antibiotics, cytostatics, fungicides, ets.), b) H+-ATPase, a plasmam membrane proton pump that plays a crucial role in the physiology of fungi, planst. The chemical stress will include compounds frequently used in medicine or agriculture, such as antibiotics, cytostatics, protonophores and fungicides. The impact of structure in different stressor analogs will be assessed. The effect of stress on cells depending on different conditions will be evaluated (an inhibitory effect, activation or inactivation of the transporters, etc.). Cells´ response to stress will be monitored with a fluorescence method using a redistribution potentiometric fluorescent probe diS-C3(3) which is a substrate of two major MDR pumps. Since there exists a similarity between the yeast MDR pumps and mambrane proteins of animals or pathogenic microorganisms responsible for resistance to antibiotics, cytostatics, etc., the results of the project can be applied in the field of medicine, enviromental protection or agriculture.

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