• FluoTRAM: Fluorescence-detected Transient Absorption Microscopy

    Grant agency: EU-MSCA
    Identification number of grant: 102-56/21546
    Principal investigator: RNDr. Pavel Malý, Ph.D.

    Grant annotation

    Fluorescence microscopy is an indispensable tool in many areas of research. In life sciences it has been perfected for biological sample imaging either by its autofluorescence or using fluorescent markers such as dyes or fluorescent proteins. It is thus possible to localize molecules in cells, obtaining information on their dynamics and environment. The fluorescence detection, by its nature, provides information only about on the final, emissive state of the molecules after photoexcitation. Meanwhile, transient absorption spectroscopy enables to track the initial state of the molecules after absorption and the following excitation dynamics. However, such ultrafast nonlinear techniques typically require volume samples and are detected coherently. We have recently developed a new way to measure transient absorption by detecting the sample fluorescence. In project FluoTRAM, we will implement our technique in the fluorescence microscope, where it truly reveals its potential. Using the established imaging techniques and markers, FluoTRAM brings the additional information on the excitation event and the dynamics towards the emissive state. This comprehensive additional information will be of great use in life sciences and beyond, examples include correlation of the excitation and emission spectra (increased Stokes shift vs red shift) for dye probes, intramolecular charge transfer in fluorescent proteins, or charge transfer and recombination in organic materials.

    For details see the webpage of the project .

    EU MSCA grant number 101030656